Journal: BMC biology

Article Title: Single-cell expression profile of Drosophila ovarian follicle stem cells illuminates spatial differentiation in the germarium.

doi: 10.1186/s12915-023-01636-9

Figure Lengend Snippet: Fig. 8 Expression patterns of selected genes. A–F Expression patterns of named gene products or derivatives in germaria co-stained with DAPI (blue in A–D) to reveal all nuclei and antibody to Fas3 (red in A–D; white in E–G) to allow location of the anterior edge of strong Fas3 expression (yellow arrows). A–A” Semaphorin 1a-GFP protein fusion (green) was detected on the surface of somatic cells; levels were low in region 1 (extreme anterior, left), peaked in region 2a ECs, FSCs, and early FCs, and declined in region 3 (posterior of the germarium) and the first budded egg chamber. B–B” Dystroglycan, detected by an antibody to the C-terminus (green), which connects the plasma membrane to ECM components, was seen lining the germarial wall from region 2a (white arrowheads in B’) and continuing posterior, consistent with its expected role and expression in all FSCs, early FCs, and a limited number of the most posterior ECs. Expression was also seen in the muscle sheath (cyan arrowheads in B). C–C” A Plum-GFP protein fusion (green) encoded by a large BAC genomic transgene was expressed most prominently in somatic cells, with highest levels in the FSC region and further posterior. D–D” A Cyclin B-GFP protein fusion showed occasional expression in layer1 FSCs (white arrow in D’ and D”) immediately anterior to the Fas3 border and was seen at similar levels in most FCs. Absence in some FCs is most likely due to protein degradation in G1 and S phases. Expression at the anterior of the germarium is within germline cells. E–G Santa Maria-Gal4 > UAS-RFP produced no RFP signal in most germaria. In other germaria, few cells expressed detectable RFP, including germaria with RFP signal in E a layer 1 FSC (white arrow) and a neighboring FC, F, G an FC adjacent to the Fas3 border, and G another early FC anterior to region 3. All bars, 20 µm

Article Snippet: Each ovary pair was then broken apart in PBS on a slide and mounted using DAPI fluoromountG mounting medium (Southern BioTech, OB010020).

Techniques: Expressing, Staining, Clinical Proteomics, Membrane, Produced

Journal: Oncotarget

Article Title: Procollagen-lysine 2-oxoglutarate 5-dioxygenase 2 promotes hypoxia-induced glioma migration and invasion

doi: 10.18632/oncotarget.15581

Figure Lengend Snippet: ( A ) Representative confocal images of U87-shNC and U87-shPLOD2 cells under normoxic and hypoxic conditions (Scale bars, 10 μm). Blue: DAPI (nuclei); red: phalloidin (polymerized actin/stress fiber); green: paxillin (focal adhesion). ( B ) Western blot for the detection of FAK-p 397 levels in U87-shNC control and U87-shPLOD2 cells under normoxic and hypoxic conditions. ( C ) Representative confocal images of U251-shNC and U251-shPLOD2 cells under normoxic and hypoxic conditions (Scale bars, 10 μm). ( D ) Western blot for the detection of FAK-p 397 levels in U251-shNC control and U251-shPLOD2 cells under normoxic and hypoxic conditions.

Article Snippet: F-actin and nuclei were stained with phalloidin (Beyotime Biotechnology) and DAPI nuclear stain (Southern Biotech; Birmingham, AL, USA), respectively.

Techniques: Western Blot, Control